Histone 3 Antikörper (pThr45)

Produktnummer ABIN6972078

Der Kaninchen Polyklonal Anti-Histone 3 Antikörper wurde für WB und DB validiert. Er ist geeignet, Histone 3 in Proben von Human und Saccharomyces cerevisiae zu detektieren.

Kurzübersicht für Histone 3 Antikörper (pThr45) (ABIN6972078)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Dot Blot (DB)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: pThr45

Produktmerkmale: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of histone H2A, histone H2B, histone H3 and histone H4). Histones are subject to a number of post-translational modifications, including acetylation, phosphorylation and methylation. In budding yeast, H3 Thr45 phosphorylation occurs specifically during DNA replication, and is catalyzed by the Cdc7-Dbf4 kinase. Yeast that lack threonine 45 exhibit defects in replication, suggesting that phosphorylation of Thr45 is associated with DNA replication. Threonine 45 lies within a conserved region of histone H3 that makes critical contacts with DNA when assembled into chromatin. It is located at the points of entry and exit of DNA on the nucleosome, and phosphorylation may influence histone-DNA interactions within the nucleosome. Histone H3T45ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot, it has been shown to react with Budding Yeast and Human samples, but it is predicted that it will react with a wide range of sample types.

Aufreinigung: Affinity Purified

Immunogen: This Histone H3 phospho Thr45 antibody was raised against a peptide containing phospho Thr45 of human histone H3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Molekulargewicht: 17 kDa

NCBI Accession: NP_003522